ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human NDUFAF5 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function
Putative methyltransferase involved in mitochondrial complex I assembly at early stages.
Recombinant NDUFAF5 Antibody (Ab117357) - Western Blot
All lanes: Recombinant NDUFAF5 Antibody (Ab117357) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 37 kDa
Exposure time: 23.1 s
Recombinant NDUFAF5 Antibody (Ab117357) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant NDUFAF5 Antibody (Ab117357). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant NDUFAF5 Antibody (Ab117357) - IF/ICC
IF analysis of Recombinant NDUFAF5 Antibody (Ab117357) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant NDUFAF5 Antibody (Ab117357) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
1/500-1/1000
1/50 - 1/100
1/200
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0506130 |
分析证书 | Ab117357 |
| ZJ24F0506131 |
分析证书 | Ab117357 |
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