Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
宿主种属
兔(Rabbit)
特异性
NDUFS2
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant protein of human NDUFS2 (AA 300-407)
阳性对照
WB: HeLa, Hep G2, U-2 OS, A431, Jurkat, RAAW 264.7 and C6 cell lysates. IHC: Human breast cancer tissue. IF/ICC: HeLa cells.
Recombinant NDUFS2 Antibody (Ab117398) - Western Blot All lanes: Recombinant NDUFS2 Antibody (Ab117398) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 53 kDa Observed band size: 45 kDa Exposure time: 23.0 s
Recombinant NDUFS2 Antibody (Ab117398) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant NDUFS2 Antibody (Ab117398). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant NDUFS2 Antibody (Ab117398) - IF/ICC IF analysis of Recombinant NDUFS2 Antibody (Ab117398) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant NDUFS2 Antibody (Ab117398) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).