Rabbit anti Human NDUFS6 Antibody, Recombinant, could be used for WB, IHC and so on. Application:
WB: 1/500-1/1000 IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
规格
产品名称
Recombinant NDUFS6 Antibody
别名
NDUFS6蛋白抗体 | 重组NDUFS6抗体
英文别名
BC059730 | CI 13kA | CI 13kD A | CI-13kD-A | CI13KDA | Complex I 13kD A | Complex I 13kDa subunit A | Complex I mitochondrial respiratory chain 13 kD subunit | Complex I-13kD-A | IP13 | NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH coenzyme
规格或纯度
ExactAb™, Validated, 重组, 0.5 mg/mL
宿主种属
兔(Rabbit)
特异性
NDUFS6
种属反应性
人(Human),小鼠(Mouse)
免疫原
Recombinant protein of human NDUFS6 (AA 29-124)
阳性对照
WB: Mouse heart, HeLa, MCF7 and Hep G2 lysates. IHC: Human tonsil tissue. IF/ICC: HeLa cells.
Recombinant NDUFS6 Antibody (Ab117416) - Western Blot All lanes: Recombinant NDUFS6 Antibody (Ab117416) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 14 kDa Observed band size: 15 kDa Exposure time: 4.3 s
Recombinant NDUFS6 Antibody (Ab117416) - Western Blot All lanes: Recombinant NDUFS6 Antibody (Ab117416) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 14 kDa Observed band size: 15 kDa Exposure time: 28.5 s
Recombinant NDUFS6 Antibody (Ab117416) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant NDUFS6 Antibody (Ab117416). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant NDUFS6 Antibody (Ab117416) - IF/ICC IF analysis of Recombinant NDUFS6 Antibody (Ab117416) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant NDUFS6 Antibody (Ab117416) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).