Rabbit anti Human NIFK Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on. Application: WB: 1/500-1/2000 IHC: 1/100-1/300 IF/ICC: 1/200-1/1000 Post-translational Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.
NIFK Antibody (Ab118085) - Western Blot All lanes: NIFK Antibody (Ab118085) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/5000 dilution Predicted band size: 34 kDa Observed band size: 31 kDa Exposure time: 150.0 s
NIFK Antibody (Ab118085) - IHC Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NIFK Antibody (Ab118085). The picture on the right is blocked with the synthesized peptide.
NIFK Antibody (Ab118085) - IF/ICC Immunofluorescence analysis of HUVEC cells, using NIFK Antibody (Ab118085). The picture on the right is blocked with the synthesized peptide.