Rabbit anti Human NME2 Antibody, Recombinant, could be used for WB, IHC and so on. Application:
WB: 1/500-1/1000 IHC: 1/50-1/100
IF/ICC: 1/200 Protein Function Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity.
规格
产品名称
Recombinant NME2 Antibody
别名
肿瘤抑制基因NME2抗体 | 重组 NME2 抗体
英文别名
C myc purine binding transcription factor PUF antibody | C myc transcription factor antibody | C-myc purine-binding transcription factor PUF antibody | epididymis secretory sperm binding protein Li 155an antibody | HEL-S-155an antibody | Histidine protein
规格或纯度
ExactAb™, Validated, 重组, 0.2 mg/mL
宿主种属
兔(Rabbit)
特异性
NME2
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human NME2 (AA 122-135)
阳性对照
WB: Mouse spleen, U-2 OS, LNCaP, HEK293, HeLa, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.
Recombinant NME2 Antibody (Ab118320) - Western Blot All lanes: Recombinant NME2 Antibody (Ab118320) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 0.2 s
Recombinant NME2 Antibody (Ab118320) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant NME2 Antibody (Ab118320). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant NME2 Antibody (Ab118320) - IF/ICC IF analysis of Recombinant NME2 Antibody (Ab118320) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant NME2 Antibody (Ab118320) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).