Rabbit anti Human Oct-2 Antibody, Recombinant, could be used for WB, IHC and so on. Application
WB: 1/500-1/2000 IHC: 1/50-1/200
IF/ICC: 1/50 Protein Function Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
规格
产品名称
Recombinant Oct-2 Antibody
别名
Oct2抗体 | 重组Oct-2抗体
英文别名
class 2 antibody | Lymphoid restricted immunoglobulin octamer binding protein antibody | Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody | NF A2 antibody | Oct 2 antibody | Oct-2 antibody | Octamer binding transcription factor 2
规格或纯度
重组, ExactAb™, 已验证, 见COA
宿主种属
兔(Rabbit)
特异性
Oct-24
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Oct-2 (AA 1-479).
阳性对照
WB: Ramos and Raji cell lysates. IHC: Human tonsil tissue. IF/ICC: HeLa cells.
Recombinant Oct-2 Antibody (Ab119151) - Western Blot All lanes: Recombinant Oct-2 Antibody (Ab119151) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 51 kDa Observed band size: 57 kDa Exposure time: 100.0 s
Recombinant Oct-2 Antibody (Ab119151) - IHC Immunohistochemical analysis of paraffin-embedded Human tonsil, using Recombinant Oct-2 Antibody (Ab119151) at 1/50 dilution.
Recombinant Oct-2 Antibody (Ab119151) - IF/ICC IF analysis of Recombinant Oct-2 Antibody (Ab119151) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Oct-2 Antibody (Ab119151) at 1/50 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).