Rabbit anti Human p18 INK4c/CDKN2C Antibody, Recombinant, could be used for WB, IHC and so on. Application:
WB: 1/500 - 1/1000 IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB.
Recombinant p18 INK4c/CDKN2C Antibody (Ab119609) - Western Blot All lanes: Recombinant p18 INK4c/CDKN2C Antibody (Ab119609) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 50.0 s
Recombinant p18 INK4c/CDKN2C Antibody (Ab119609) - IF/ICC IF analysis of Recombinant p18 INK4c/CDKN2C Antibody (Ab119609) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant p18 INK4c/CDKN2C Antibody (Ab119609) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).