Rabbit anti Human p27 KIP 1 Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on. Application WB: 1/500-1/2000 IHC: 1/50-1/200 ICC/IF: 1/50-1/200 Protein Function Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Recombinant p27 KIP 1 Antibody (Ab119643) - Western Blot All lanes: Recombinant p27 KIP 1 Antibody (Ab119643) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 22 kDa Observed band size: 27 kDa Exposure time: 80.0 s
Recombinant p27 KIP 1 Antibody (Ab119643) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant p27 KIP 1 Antibody (Ab119643).
Recombinant p27 KIP 1 Antibody (Ab119643) - IHC Immunohistochemical analysis of paraffin-embedded human colon, using Recombinant p27 KIP 1 Antibody (Ab119643).