Rabbit anti Human MAPK11 Antibody, Polyclonal antibody, could be used for WB, IHC and so on. Application WB: 1/500 - 1/1000 IHC: 1/50 - 1/100 Protein Function Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Phosphorylates preferentially transcription factor ATF2. Post-translational Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
MAPK11 Antibody (Ab119724) - Western Blot All lanes: MAPK11 Antibody (Ab119724) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 60.0 s
MAPK11 Antibody (Ab119724) - Western Blot All lanes: MAPK11 Antibody (Ab119724) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa Observed band size: 39 kDa Exposure time: 100.0 s
MAPK11 Antibody (Ab119724) - IHC Immunohistochemistry analysis of paraffin-embedded Human brain using MAPK11 Antibody (Ab119724). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
MAPK11 Antibody (Ab119724) - IF/ICC
IF analysis of MAPK11 Antibody (Ab119724) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with MAPK11 Antibody (Ab119724) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ23F1202092
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