Rabbit anti Human p46 Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA.
规格
产品名称
p46 Antibody
别名
DNA聚合酶δ相互作用蛋白3抗体 | p46 抗体
英文别名
46 kDa DNA polymerase delta interaction protein | p46 | S6K1 Aly/REF-like target | SKAR | 6 kDa DNA polymerase delta interaction protein antibody | DNA polymerase delta interacting protein 3 antibody | KIAA1649 antibody | P46 antibody | PDIP3_HUMAN antibo
规格或纯度
ExactAb™, Validated, Carrier Free, 0.6 mg/mL
宿主种属
兔(Rabbit)
特异性
POLDIP3
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Fusion protein of human POLDIP3 (AA 36-229)
阳性对照
WB: HeLa, Hep G2, Jurkat, HEK293, RAW 264.7 and C6 cell lysates. IHC: Human thyroid cancer tissue. IF/ICC: HeLa cells.
p46 Antibody (Ab119753) - Western Blot All lanes: p46 Antibody (Ab119753) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 46 kDa Observed band size: 44, 47 kDa Exposure time: 55.2 s
p46 Antibody (Ab119753) - Western Blot All lanes: p46 Antibody (Ab119753) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 46 kDa Observed band size: 44, 47 kDa Exposure time: 73.3 s
p46 Antibody (Ab119753) - IHC Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using p46 Antibody (Ab119753). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
p46 Antibody (Ab119753) - IF/ICC
IF analysis of p46 Antibody (Ab119753) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with p46 Antibody (Ab119753) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).