Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
宿主种属
兔(Rabbit)
特异性
p95/NBS1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human p95/NBS1 (AA 680-754)
阳性对照
WB: HeLa, Jurkat, A549, K562, MCF7, NIH/3T3 and C6 cell lysates. IHC: Rat kidney, heart, Human lung large cell cancer, glioblastoma, Mouse heart tissues. IF/ICC: HeLa cells.
Recombinant p95/NBS1 Antibody (Ab119962) - Western Blot All lanes: Recombinant p95/NBS1 Antibody (Ab119962) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 85 kDa Observed band size: 91 kDa Exposure time: 20.0 s
Recombinant p95/NBS1 Antibody (Ab119962) - IF/ICC Immunofluorescent analysis of HeLa, using the Recombinant p95/NBS1 Antibody (Ab119962) at 1/50 dilution.
Recombinant p95/NBS1 Antibody (Ab119962) - IHC Immunohistochemical analysis of paraffin-embedded Rat kidney, using the Recombinant p95/NBS1 Antibody (Ab119962) at 1/100 dilution.
Recombinant p95/NBS1 Antibody (Ab119962) - IHC Immunohistochemical analysis of paraffin-embedded Rat heart, using the Recombinant p95/NBS1 Antibody (Ab119962) at 1/100 dilution.
Recombinant p95/NBS1 Antibody (Ab119962) - IHC Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Recombinant p95/NBS1 Antibody (Ab119962) at 1/100 dilution.
Recombinant p95/NBS1 Antibody (Ab119962) - IHC Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Recombinant p95/NBS1 Antibody (Ab119962) at 1/100 dilution.
Recombinant p95/NBS1 Antibody (Ab119962) - IHC Immunohistochemical analysis of paraffin-embedded Mouse heart, using the Recombinant p95/NBS1 Antibody (Ab119962) at 1/100 dilution.
Recombinant p95/NBS1 Antibody (Ab119962) - IHC Immunohistochemical analysis of paraffin-embedded rat heart, using Recombinant p95/NBS1 Antibody (Ab119962).
ZJ25F0117215
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