Rabbit anti Human PABPN1 Antibody, Recombinant, could be used for WB and IHC. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation.
Recombinant PABPN1 Antibody (Ab119979) - Western Blot All lanes: Recombinant PABPN1 Antibody (Ab119979) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 50 kDa Observed band size: 49 kDa
Recombinant PABPN1 Antibody (Ab119979) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using PABPN1 antibody (Ab119979) at 1/50 dilution. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.