Rabbit anti Human PAK1 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500 - 1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Post-translational
Autophosphorylated when activated by CDC42/p21 and RAC1.
Recombinant PAK1 Antibody (Ab120108) - Western Blot All lanes: Recombinant PAK1 Antibody (Ab120108) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 12.8 s
Recombinant PAK1 Antibody (Ab120108) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant PAK1 Antibody (Ab120108). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant PAK1 Antibody (Ab120108) - IF/ICC Immunocytochemistry analysis of PAK1 (green) in 3T3 using Recombinant PAK1 Antibody (Ab120108) and DAPI (blue).