ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human PAK2 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500 - 1/1000
IHC: 1/50 - 1/100
IF/ICC: 1/200
Protein Function
The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.
Post-translational
Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.
During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.
Ubiquitinated, leading to its proteasomal degradation.
PAK-2p34 is myristoylated.
Recombinant PAK2 Antibody (Ab120114) - Western Blot
All lanes: Recombinant PAK2 Antibody (Ab120114) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 59 kDa
Exposure time: 10.1 s
Recombinant PAK2 Antibody (Ab120114) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant PAK2 Antibody (Ab120114). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant PAK2 Antibody (Ab120114) - IF/ICC
IF analysis of Recombinant PAK2 Antibody (Ab120114) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant PAK2 Antibody (Ab120114) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
1/500-1/1000
1/50 - 1/100
1/200
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0606455 |
分析证书 | Ab120114 |
| ZJ24F0606456 |
分析证书 | Ab120114 |
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