ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human pan Cadherin Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500 - 1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 |
Relevance
Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis.
Recombinant pan Cadherin Antibody (Ab120139) - Western Blot
All lanes: Recombinant pan Cadherin Antibody (Ab120139) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 97 kDa
Observed band size: 100-140 kDa
Exposure time: 4.1 s
Recombinant pan Cadherin Antibody (Ab120139) - Western Blot
All lanes: Recombinant pan Cadherin Antibody (Ab120139) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 97 kDa
Observed band size: 124 kDa
Exposure time: 12.2 s
Recombinant pan Cadherin Antibody (Ab120139) - IF/ICC
Immunocytochemistry analysis of pan Cadherin (green) in MCF7 using Recombinant pan Cadherin Antibody (Ab120139), and cells were counterstained with DAPI (blue).
Recombinant pan Cadherin Antibody (Ab120139) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant pan Cadherin Antibody (Ab120139). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
1/500-1/1000
1/50 - 1/100
1/50-1/200
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0506146 |
分析证书 | Ab120139 |
| ZJ24F0506147 |
分析证书 | Ab120139 |
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