Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
宿主种属
兔(Rabbit)
特异性
PARP1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human PARP1 (AA 1-50)
阳性对照
WB: K562, MCF7 and HEK293 cell ysates. IHC: Human testis tissue. IF/ICC: HeLa cells.
Recombinant PARP1 Antibody (Ab120377) - Western Blot All lanes: Recombinant PARP1 Antibody (Ab120377) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 113 kDa Observed band size: 117 kDa Exposure time: 56.3 s
Recombinant PARP1 Antibody (Ab120377) - Western Blot All lanes: Recombinant PARP1 Antibody (Ab120377) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 113 kDa Observed band size: 89, 117 kDa Exposure time: 100.0 s
Recombinant PARP1 Antibody (Ab120377) - IHC Immunohistochemical analysis of paraffin-embedded human testis, using Recombinant PARP1 Antibody (Ab120377).
Recombinant PARP1 Antibody (Ab120377) - IF/ICC Immunofluorescent analysis of Hela cells, using Recombinant PARP1 Antibody (Ab120377).
ZJ25F0623376
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