Pea3 Antibody (Ab121132) - Western Blot All lanes: Pea3 Antibody (Ab121132) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 53 kDa Observed band size: 57 kDa Exposure time: 100.0 s
Pea3 Antibody (Ab121132) - IHC Immunohistochemical analysis of ETV4 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Pea3 Antibody (Ab121132) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.