Rabbit anti Human PFAS Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 1/500-1/2000
IHC: 1/50-1/300
IF/ICC: 1/200 Relevance Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. PFAS catalyzes the fourth step of IMP biosynthesis.
PFAS Antibody (Ab121397) - Western Blot All lanes: PFAS Antibody (Ab121397) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 145 kDa Observed band size: 133 kDa Exposure time: 120.1 s
PFAS Antibody (Ab121397) - IHC Immunohistochemistry analysis of paraffin-embedded human tonsil labelling PFAS with PFAS Antibody (Ab121397) at 1/200 dilution (4℃, overnight). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was used for antigen retrieval. Secondary antibody was diluted at 1/200 dilution (room temperature, 45min).
PFAS Antibody (Ab121397) - IF/ICC
IF analysis of PFAS Antibody (Ab121397) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PFAS Antibody (Ab121397) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).
ZJ24F0910285
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