WB: HeLa, HEK293, A431, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human cerebrum, liver, kidney, stomach, breast cancer, prostate tissues. IF/ICC: HeLa cells.
PGM1 Antibody (Ab121491) - Western Blot All lanes: PGM1 Antibody (Ab121491) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 65.9 s
PGM1 Antibody (Ab121491) - IHC DAB staining on IHC Samples: Human Cerebrum Tissue Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PGM1 Antibody (Ab121491) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PGM1 Antibody (Ab121491) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PGM1 Antibody (Ab121491) - IHC DAB staining on IHC Samples: Human Stomach Tissue Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PGM1 Antibody (Ab121491) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PGM1 Antibody (Ab121491) - IHC DAB staining on IHC Samples: Human Prostate Tissue Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PGM1 Antibody (Ab121491) - IHC DAB staining on IHC Sample: Human Liver Tissue Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PGM1 Antibody (Ab121491) - IHC DAB staining on IHC Sample: Human Prostate Tissue Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PGM1 Antibody (Ab121491) - IHC DAB staining on IHC Sample: Human Kidney Tissue Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PGM1 Antibody (Ab121491) - IF/ICC IF analysis of PGM1 Antibody (Ab121491) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PGM1 Antibody (Ab121491) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).