Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism.
宿主种属
兔(Rabbit)
特异性
EGLN3
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant protein of human PHD3 (AA 1-239)
阳性对照
WB: Mouse lung, HeLa, A549, MCF7, NIH/3T3 and C6 lysates. IHC: Human colon cancer tissue. IF/ICC: HeLa cells.
Recombinant PHD3 Antibody (Ab121571) - Western Blot All lanes: Recombinant PHD3 Antibody (Ab121571) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 27 kDa Observed band size: 29 kDa Exposure time: 58.5 s
Recombinant PHD3 Antibody (Ab121571) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant PHD3 Antibody (Ab121571). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant PHD3 Antibody (Ab121571) - IF/ICC IF analysis of Recombinant PHD3 Antibody (Ab121571) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant PHD3 Antibody (Ab121571) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
ZJ24F0709039
危险品化学品经营许可证（带存储）