Rabbit anti Human PIM1 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function: May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis.
Recombinant PIM1 Antibody (Ab121877) - Western Blot All lanes: Recombinant PIM1 Antibody (Ab121877) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 36 kDa Observed band size: 32 kDa Exposure time: 30.0 s
Recombinant PIM1 Antibody (Ab121877) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant PIM1 Antibody (Ab121877). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant PIM1 Antibody (Ab121877) - IF/ICC
IF analysis of Recombinant PIM1 Antibody (Ab121877) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant PIM1 Antibody (Ab121877) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ24F0202766
危险品化学品经营许可证（带存储）