Rabbit anti Human PKC alpha Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/200 Protein Function This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Recombinant PKC alpha Antibody (Ab122024) - Western Blot All lanes: Recombinant PKC alpha Antibody (Ab122024) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 3.4 s
Recombinant PKC alpha Antibody (Ab122024) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant PKC alpha Antibody (Ab122024) at 1/50 dilution. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
IF analysis of Recombinant PKC alpha Antibody (Ab122024) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant PKC alpha Antibody (Ab122024) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).
ZJ24F0911294
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