Rabbit anti Human PKR Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function: Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
规格
产品名称
Recombinant PKR Antibody
别名
蛋白激酶R(PKR)抗体 | PKR抗体 | 重组 PKR 抗体
英文别名
2.7.10.2 | EC 2.7.11.1 | eIF-2A protein kinase 2 | Eukaryotic translation initiation factor 2-alpha kinase 2 | Interferon-inducible RNA-dependent protein kinase | P1/eIF-2A protein kinase | p68 kinase | PKR | Protein kinase R | Protein kinase RNA-activate
规格或纯度
ExactAb™, Validated, 重组, 0.2 mg/mL
宿主种属
兔(Rabbit)
特异性
PKR
种属反应性
人(Human)
免疫原
A synthetic peptide derived from human PKR (AA 508-526)
阳性对照
WB: K562, Hep G2 and HeLa cell lysates. IHC: Human cholangiocarcinoma tissue. ICC/IF: HeLa cells.
Recombinant PKR Antibody (Ab122177) - Western Blot All lanes: Recombinant PKR Antibody (Ab122177) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 62 kDa Observed band size: 67 kDa Exposure time: 22.9 s
Recombinant PKR Antibody (Ab122177) - IF/ICC Immunocytochemistry analysis of PKR (green) in HeLa using Recombinant PKR Antibody (Ab122177), and DAPI (blue).
Recombinant PKR Antibody (Ab122177) - IHC Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant PKR Antibody (Ab122177). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.