Rabbit anti Human PLA1A/NMD Antibody, Polyclonal, could be used for WB, IHC and so on. Application:
WB: 1/500-1/2000 IHC: 1/100-1/300
IF/ICC: 1/500 Protein Function Hydrolyzes the ester bond at the sn-1 position of glycerophospholipids and produces 2-acyl lysophospholipids. Hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1-acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) or phosphatidylinositol (PI). Isoform 2 hydrolyzes lyso-PS but not PS. Hydrolysis of lyso-PS in peritoneal mast cells activated by receptors for IgE leads to stimulate histamine production.
PLA1A/NMD Antibody (Ab122182) - Western Blot All lanes: PLA1A/NMD Antibody (Ab122182) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 45 kDa Exposure time: 25.6 s
PLA1A/NMD Antibody (Ab122182) - IHC Immunohistochemistry analysis of paraffin-embedded human brain, using PLA1A/NMD Antibody (Ab122182). The picture on the right is blocked with the synthesized peptide.
PLA1A/NMD Antibody (Ab122182) - IF/ICC IF analysis of PLA1A/NMD Antibody (Ab122182) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PLA1A/NMD Antibody (Ab122182) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab138205) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).