ExactAb™,已验证,无叠氮钠,无载体,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human Plastin L Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 0.01-2µg/mL
IHC: 5-20µg/mL
Function
Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
Post-translational
Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections. The N-terminus is blocked.
Plastin L Antibody (Ab122265) - Western Blot
All lanes: Plastin L Antibody (Ab122265) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 12.3 s
Plastin L Antibody (Ab122265) - IHC
DAB staining on IHC
Sample: Human Liver Tissue
Primary Ab: 10 µg/mL Plastin L Antibody (Ab122265)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Plastin L Antibody (Ab122265) - IHC
DAB staining on IHC
Sample: Human Liver cancer Tissue
Primary Ab: 10 µg/mL Plastin L Antibody (Ab122265)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Plastin L Antibody (Ab122265) - IF/ICC
IF analysis of Plastin L Antibody (Ab122265) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Plastin L Antibody (Ab122265) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
5-20 µg/mL
0.01-2 µg/mL
2.5 µg/mL
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0404402 |
分析证书 | Ab122265 |
| ZJ24F0404403 |
分析证书 | Ab122265 |
| ZJ24F0404404 |
分析证书 | Ab122265 |
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