Rabbit anti Human PPP2R1A Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/3000 IHC: 1/50-1/100 Protein Function The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.
规格
产品名称
PPP2R1A Antibody
别名
蛋白质磷酸酶2调节亚基1A抗体 | PPP2R1A 抗体
英文别名
2AAA_HUMAN antibody | 6330556D22Rik antibody | Alpha isoform of regulatory subunit A, protein phosphatase antibody | DKFZp470L0914 antibody | Medium tumor antigen-associated 61 kDa protein antibody | MGC128399 antibody | MGC786 antibody | MGC83000 antibod
规格或纯度
ExactAb™, Validated, Carrier Free, High performance, 1.14 mg/mL
宿主种属
兔(Rabbit)
特异性
PPP2R1A
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Fusion protein of human PPP2R1A (AA 500-580)
阳性对照
WB: C6, Jurkat and HEK293 cell lysates. IHC: Human liver cancer lysates. IF/ICC: HeLa cells.
PPP2R1A Antibody (Ab122840) - Western Blot All lanes: PPP2R1A Antibody (Ab122840) at 1/3000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 65 kDa Observed band size: 60 kDa Exposure time: 5.0 s
PPP2R1A Antibody (Ab122840) - IHC Immunohistochemistry analysis of paraffin-embedded Human liver cancer using PPP2R1A Antibody (Ab122840). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
PPP2R1A Antibody (Ab122840) - IF/ICC
IF analysis of PPP2R1A Antibody (Ab122840) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PPP2R1A Antibody (Ab122840) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ24F0102670
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