Rabbit anti Human PPP2R4 Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine-protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+) (By similarity). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activty of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A.
PPP2R4 Antibody (Ab122864) - Western Blot All lanes: PPP2R4 Antibody (Ab122864) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 41 kDa Observed band size: 43 kDa Exposure time: 4.1 s
PPP2R4 Antibody (Ab122864) - Western Blot All lanes: PPP2R4 Antibody (Ab122864) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 41 kDa Observed band size: 36, 43 kDa Exposure time: 1.0 s
PPP2R4 Antibody (Ab122864) - IHC Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using PPP2R4 Antibody (Ab122864). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
PPP2R4 Antibody (Ab122864) - IF/ICC
IF analysis of PPP2R4 Antibody (Ab122864) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PPP2R4 Antibody (Ab122864) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).