Rabbit anti Human PRC1 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function: Cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.
Recombinant PRC1 Antibody (Ab122957) - Western Blot All lanes: Recombinant PRC1 Antibody (Ab122957) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 72 kDa Observed band size: 56, 72, 79 kDa. Recombinant PRC1 Antibody (Ab122957) recognizes two isoforms of PRC1. But we are unsure how to define the extra bands at ~50 kDa. Exposure time: 14.5 s
Recombinant PRC1 Antibody (Ab122957) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant PRC1 Antibody (Ab122957). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant PRC1 Antibody (Ab122957) - IF/ICC
IF analysis of Recombinant PRC1 Antibody (Ab122957) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant PRC1 Antibody (Ab122957) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
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