Rabbit anti Human Prion protein PrP Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/2000 IHC: 1/50-1/100 Protein Function: The function of PrP is still under debate. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis (By similarity). Isoform 2 may act as a growth suppressor by arresting the cell cycle at the G0/G1 phase. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro).
规格
产品名称
Recombinant Prion protein PrP Antibody
别名
Prion protein PrP抗体 | 朊病毒蛋白抗体 | 重组朊病毒蛋白 PrP 抗体
英文别名
Alternative prion protein | major prion protein antibody | AltPrP antibody | ASCR antibody | CD230 antibody | CD230 antigen antibody | CJD antibody | GSS antibody | KURU antibody | p27 30 antibody | PRIO_HUMAN antibody | Prion protein antibody | Prion rel
规格或纯度
ExactAb™, Validated, 重组, 1.0 mg/mL
宿主种属
兔(Rabbit)
特异性
Prion protein PrP
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Prion protein PrP (AA 214-230)
阳性对照
WB: Mouse brain, A375 and C6 lysates. IHC: Human breast cancer tissue.
Recombinant Prion protein PrP Antibody (Ab123058) - Western Blot All lanes: Recombinant Prion protein PrP Antibody (Ab123058) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 28 kDa Observed band size: 25-35 kDa Exposure time: 12.1 s
Recombinant Prion protein PrP Antibody (Ab123058) - Western Blot All lanes: Recombinant Prion protein PrP Antibody (Ab123058) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 28 kDa Observed band size: 25-35 kDa Exposure time: 10.0 s
Recombinant Prion protein PrP Antibody (Ab123058) - Western Blot All lanes: Recombinant Prion protein PrP Antibody (Ab123058) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 28 kDa Observed band size: 20-37 kDa Exposure time: 57.5 s
Recombinant Prion protein PrP Antibody (Ab123058) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant Prion protein PrP Antibody (Ab123058). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.