ExactAb™,已验证,无叠氮钠,无载体,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human Prohibitin Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 0.01-2 µg/mL
IHC: 5-30 µg/mL
IF/ICC: 2.5 µg/mL
Protein Function:
Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Prohibitin Antibody (Ab123227) - Western Blot
All lanes: Prohibitin Antibody (Ab123227) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 28 kDa
Exposure time: 14.8 s
Prohibitin Antibody (Ab123227) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC
DAB staining on IHC
Samples: Human Rectum Tissue
Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC
DAB staining on IHC
Samples: Human Thyroid cancer Tissue
Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC
DAB staining on IHC
Sample: Mouse Cardiac Muscle Tissue
Primary Ab: 30 µg/mL Prohibitin Antibody (Ab123227)
Control: Used PBS instead of primary antibody
Second Ab: 2 μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IF/ICC
IF analysis of Prohibitin Antibody (Ab123227) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Prohibitin Antibody (Ab123227) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
5-30 µg/mL
0.01-2 µg/mL
1/200
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0404499 |
分析证书 | Ab123227 |
| ZJ24F0404500 |
分析证书 | Ab123227 |
| ZJ24F0404501 |
分析证书 | Ab123227 |
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