Rabbit anti Human Proteasome 20S beta 6 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 0.01-3 µg/mL
IHC: 5-30 µg/mL
IF/ICC: 2.5 µg/mL
Protein Function:
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the peptidyl glutamyl-like activity. May catalyze basal processing of intracellular antigens.
WB: HeLa, MCF7, K562, A549 and HEK293 cell lysates. IHC: Human breast cancer, liver, kidney, pancreas, cerebrum, colorectal cancer tissues. IF/ICC: HeLa cells.
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
亚型
Rabbit IgG
纯化方法
Antigen affinity purified + Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂
0.05% Proclin 300
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
图片
Proteasome 20S beta 6 Antibody (Ab123385) - Western Blot All lanes: Proteasome 20S beta 6 Antibody (Ab123385) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size:25 kDa Observed band size: 23 kDa Exposure time: 15.7 s
Proteasome 20S beta 6 Antibody (Ab123385) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 30 µg/mL Proteasome 20S beta 6 Antibody (Ab123385) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Proteasome 20S beta 6 Antibody (Ab123385) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 30 µg/mL Proteasome 20S beta 6 Antibody (Ab123385) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Proteasome 20S beta 6 Antibody (Ab123385) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 30 µg/mL Proteasome 20S beta 6 Antibody (Ab123385) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Proteasome 20S beta 6 Antibody (Ab123385) - IHC DAB staining on IHC Samples: Human Pancreas Tissue Primary Ab: 30 µg/mL Proteasome 20S beta 6 Antibody (Ab123385) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Proteasome 20S beta 6 Antibody (Ab123385) - IHC DAB staining on IHC Samples: Human Cerebrum Tissue Primary Ab: 30 µg/mL Proteasome 20S beta 6 Antibody (Ab123385) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Proteasome 20S beta 6 Antibody (Ab123385) - IHC DAB staining on IHC Samples: Human Colorectal cancer Tissue Primary Ab: 30 µg/mL Proteasome 20S beta 6 Antibody (Ab123385) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
IF analysis of Proteasome 20S beta 6 Antibody (Ab123385) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Proteasome 20S beta 6 Antibody (Ab123385) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).