Rabbit anti Human PSMA4 Antibody, Recombinant, could be used for WB, IHC and so on. Application:
WB: 1/500-1/1000 IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Recombinant PSMA4 Antibody (Ab123653) - Western Blot All lanes: Recombinant PSMA4 Antibody (Ab123653) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 14.8 s
Recombinant PSMA4 Antibody (Ab123653) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant PSMA4 Antibody (Ab123653). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant PSMA4 Antibody (Ab123653) - IF/ICC IF analysis of Recombinant PSMA4 Antibody (Ab123653) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant PSMA4 Antibody (Ab123653) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).