Rabbit anti Human PSMB10/MECL1 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.
Recombinant PSMB10/MECL1 Antibody (Ab123661) - Western Blot All lanes: Recombinant PSMB10/MECL1 Antibody (Ab123661) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 29 kDa Observed band size: 26, 29 kDa Exposure time: 66.8 s
Recombinant PSMB10/MECL1 Antibody (Ab123661) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant PSMB10/MECL1 Antibody (Ab123661). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
IF analysis of Recombinant PSMB10/MECL1 Antibody (Ab123661) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant PSMB10/MECL1 Antibody (Ab123661) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ24F0303441
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