Rabbit anti Human PSMF1 Antibody, Polyclonal antibody, could be used for WB, IHC and so on. Application WB: 1/500 - 1/1000 IHC: 1/50-1/100
IF/ICC: 1/200 Protein Function Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.
PSMF1 Antibody (Ab123731) - Western Blot All lanes: PSMF1 Antibody (Ab123731) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 30 kDa Observed band size: 31 kDa Exposure time: 11.0 s
PSMF1 Antibody (Ab123731) - IHC Immunohistochemistry analysis of paraffin-embedded Human liver cancer using PSMF1 Antibody (Ab123731). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
PSMF1 Antibody (Ab123731) - IF/ICC IF analysis of PSMF1 Antibody (Ab123731) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PSMF1 Antibody (Ab123731) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).