Rabbit anti Human PTBP1 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
Protein Function:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/1000
Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
Recombinant PTBP1 Antibody (
Ab123766
) - Western Blot All lanes: Recombinant PTBP1 Antibody (Ab123766) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 57 kDa Observed band size: 60 kDa Exposure time: 5.9 s
Recombinant PTBP1 Antibody (Ab123766) - Western Blot All lanes: Recombinant PTBP1 Antibody (Ab123766) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 57 kDa Observed band size: 60 kDa Exposure time: 60.0 s
Recombinant PTBP1 Antibody (Ab123766) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant PTBP1 Antibody (Ab123766). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant PTBP1 Antibody (Ab123766) - IF/ICC
IF analysis of Recombinant PTBP1 Antibody (Ab123766) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant PTBP1 Antibody (Ab123766) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).