Rabbit anti Human PYGL Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 0.01-5 µg/mL
IHC: 5-20 µg/mL
IF/ICC: 2.35 µg/mL
Protein Function:
Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
规格
产品名称
PYGL Antibody
别名
PYGL抗体 | 糖原磷酸化酶L抗体 | PYGL抗体
英文别名
EC 2.4.1.1 | Glycogen phosphorylase antibody | Glycogen phosphorylase L antibody | Glycogen phosphorylase liver antibody | Glycogen phosphorylase liver form antibody | GSD6 antibody | Hers disease, glycogen storage disease type VI antibody | liver form an
规格或纯度
无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属
兔(Rabbit)
特异性
PYGL
种属反应性
人(Human),大鼠(Rat)
免疫原
Recombinant PYGL expressed in E.coli (AA 370-538).
阳性对照
WB: HeLa and HEK293 cell lysates. IHC: Human liver, liver cancer, kidney, Mouse liver tissues. IF/ICC: HeLa cells.
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
亚型
Rabbit IgG
纯化方法
Antigen affinity purified + Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂
0.05% Proclin 300
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
图片
PYGL Antibody (Ab124003) - Western Blot All lanes: PYGL Antibody (Ab124003) at 1/5000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 97 kDa Observed band size: 93 kDa Exposure time: 60.9 s
PYGL Antibody (Ab124003) - Western Blot All lanes: PYGL Antibody (Ab124003) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 97 kDa Observed band size: 93 kDa Exposure time: 1.0 s
PYGL Antibody (Ab124003) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 20 µg/mL PYGL Antibody (Ab124003) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PYGL Antibody (Ab124003) - IHC DAB staining on IHC Samples: Human Liver cancer Tissue Primary Ab: 20 µg/mL PYGL Antibody (Ab124003) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PYGL Antibody (Ab124003) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 20 µg/mL PYGL Antibody (Ab124003) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PYGL Antibody (Ab124003) - IHC DAB staining on IHC Sample: Mouse Liver Tissue Primary Ab: 20 μg/mL PYGL Antibody (Ab124003) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PYGL Antibody (Ab124003) - IF/ICC
IF analysis of PYGL Antibody (Ab124003) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PYGL Antibody (Ab124003) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).