Rabbit anti Human PTK2B Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function
Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
规格
产品名称
Recombinant PYK2 Antibody
别名
兔单克隆抗体 to PYK2 | 蛋白酪氨酸激酶2β(PYK2)单克隆抗体 | PTK2B抗体 | 重组PYK2抗体
Recombinant PYK2 Antibody (Ab124023) - Western Blot All lanes: Recombinant PYK2 Antibody (Ab124023) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 116 kDa Observed band size: 109 kDa Exposure time: 28.2 s
Recombinant PYK2 Antibody (Ab124023) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon using Recombinant PYK2 Antibody (Ab124023). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant PYK2 Antibody (Ab124023) - IF/ICC
IF analysis of Recombinant PYK2 Antibody (Ab124023) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant PYK2 Antibody (Ab124023) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).