Rabbit anti Human Quinone oxidoreductase Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 1/500-1/2000 IHC: 1/50-1/300
IF/ICC: 1/500 Protein Function Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding.
Quinone oxidoreductase Antibody (Ab124077) - Western Blot All lanes: Quinone oxidoreductase Antibody (Ab124077) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 36 kDa Observed band size: 35 kDa Exposure time: 17.7 s
Quinone oxidoreductase Antibody (Ab124077) - IHC Immunohistochemical analysis of paraffin-embedded human liver cancer. Quinone oxidoreductase Antibody (Ab124077) was diluted at 1/200 (4°C overnight). Tris-EDTA, pH 9.0 was used for antigen retrieval. Secondary antibody was diluted at 1/200 (room temperature, 45 min).
Quinone oxidoreductase Antibody (Ab124077) - IF/ICC IF analysis of Quinone oxidoreductase Antibody (Ab124077) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Quinone oxidoreductase Antibody (Ab124077) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab138205) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).