ExactAb™,已验证,无载体,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Mouse anti Human RAB1B Antibody, Monoclonal (1673CT667.16.73), could be used for WB, Flow and so on.
Application
WB: 1/4000
Flow: 1/25
Protein Function
Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.
Post-translational
Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1). AMPylation at Tyr-77 occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB1B constitutively active.
RAB1B Mouse Antibody (Ab124143) - Western Blot
All lanes: RAB1B Mouse Antibody (Ab124143) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/5000 dilution
Predicted band size: 22 kDa
Observed band size: 23 kDa
Exposure time: 80.4 s
RAB1B Mouse Antibody (Ab124143) - Flow Cytometry
Overlay histogram showing A431 cells stained with RAB1B Mouse Antibody (Ab124143) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1 x 10^6 cells) used under the same conditions.
Acquisition of >10, 000 events was performed.
1/25
1/4000
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0910063 |
分析证书 | Ab124143 |
| ZJ24F0910064 |
分析证书 | Ab124143 |
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