ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human RAB3GAP1 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/50
Protein Function
Probable catalytic subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Specifically converts active Rab3-GTP to the inactive form Rab3-GDP. Required for normal eye and brain development. May participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters.
RAB3GAP1 Antibody (Ab124213) - Western Blot
All lanes: RAB3GAP1 Antibody (Ab124213) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 110 kDa
Observed band size: 125 kDa
Exposure time: 120.0 s
RAB3GAP1 Antibody (Ab124213) - IHC
Immunohistochemical analysis of RAB3GAP1 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with RAB3GAP1 Antibody (Ab124213) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
RAB3GAP1 Antibody (Ab124213) - IF/ICC
IF analysis of RAB3GAP1 Antibody (Ab124213) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with RAB3GAP1 Antibody (Ab124213) at 1/50 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
1/50 - 1/100
1/500-1/1000
1/50
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0910703 |
分析证书 | Ab124213 |
| ZJ24F0910704 |
分析证书 | Ab124213 |
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