Recombinant Rab9 Antibody (Ab124272) - Western Blot All lanes: Recombinant Rab9 Antibody (Ab124272) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 23.1 s
Recombinant Rab9 Antibody (Ab124272) - Western Blot All lanes: Recombinant Rab9 Antibody (Ab124272) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 10.0 s
Recombinant Rab9 Antibody (Ab124272) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant Rab9 Antibody (Ab124272). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant Rab9 Antibody (Ab124272) - IF/ICC IF analysis of Recombinant Rab9 Antibody (Ab124272) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Rab9 Antibody (Ab124272) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).