Rabbit anti Human Rad50 Antibody, Recombinant, could be used for WB, IHC and so on. Application: · WB: 1/500-1/1000 · IHC: 1/50-1/100 Protein Function Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
规格
产品名称
Recombinant Rad50 Antibody
别名
DNA修复蛋白RAD50抗体 | 兔单克隆抗体 to Rad50 | 重组Rad50抗体
英文别名
DNA repair protein RAD50 antibody | HRAD50 antibody | HsRAD50 antibody | Mrell antibody | NBSLD antibody | Rad 50 antibody | RAD50 2antibody | rad50 antibody | RAD50 homolog (S cerevisiae) antibody | RAD50 homolog antibody | RAD50 PEN antibody | RAD50, S.
规格或纯度
ExactAb™, Validated, 重组, 0.6 mg/mL
宿主种属
兔(Rabbit)
特异性
RAD50
种属反应性
人(Human)
免疫原
Recombinant protein of human Rad50 (AA 1-425).
阳性对照
WB: MOLT-4, Jurkat and K562 cell lysates. IHC: Human Cholangiocarcinoma.
Recombinant Rad50 Antibody (Ab124364) - Western Blot All lanes: Recombinant Rad50 Antibody (Ab124364) at 1/1500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 153 kDa Observed band size: 151 kDa Exposure time: 5.0 s
Recombinant Rad50 Antibody (Ab124364) - IHC Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma tissue using Recombinant Rad50 Antibody (Ab124364). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.