ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human RAP80 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function:
Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
Recombinant RAP80 Antibody (Ab124603) - Western Blot
All lanes: Recombinant RAP80 Antibody (Ab124603) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDa
Exposure time: 15.9 s
Recombinant RAP80 Antibody (Ab124603) - IF/ICC
Immunocytochemistry analysis of RAP80 (green) in HeLa using Recombinant RAP80 Antibody (Ab124603), and cells were counterstained with DAPI (blue).
Recombinant RAP80 Antibody (Ab124603) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Recombinant RAP80 Antibody (Ab124603). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
1/500-1/1000
1/50 - 1/100
1/50-1/200
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0404029 |
分析证书 | Ab124603 |
| ZJ24F0404030 |
分析证书 | Ab124603 |
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