Rabbit anti Human RBBP4 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/50-1/200
Protein Function
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
规格
产品名称
Recombinant RBBP4 Antibody
别名
视网膜母细胞瘤结合蛋白P48抗体 | 重组RBBP4抗体
英文别名
CAF-1 p48 | CAF-1 subunit C | CAF-1C | CAF-I p48 | Chromatin assembly factor 1 subunit C | Chromatin assembly factor I p48 subunit | chromatin assembly factor/CAF-1 p48 subunit | histone-binding protein RBBP4 | MSI1 protein homolog | Nucleosome-remodeling
规格或纯度
ExactAb™, Validated, 重组, 0.6 mg/mL
宿主种属
兔(Rabbit)
特异性
RBBP4
种属反应性
人(Human),仓鼠(Hamster),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human RbAp48 (AA 2-15)
阳性对照
WB: Mouse spleen, HeLa, Jurkat, HEK293, Hep G2, NIH/3T3 and C6 lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.
Recombinant RBBP4 Antibody (Ab124746) - Western Blot All lanes: Recombinant RBBP4 Antibody (Ab124746) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 48 kDa Observed band size: 50 kDa Exposure time: 5.0 s
Recombinant RBBP4 Antibody (Ab124746) - IF/ICC Immunocytochemistry analysis of RBBP4 (green) in HeLa using Recombinant RBBP4 Antibody (Ab124746), and DAPI (blue).
Recombinant RBBP4 Antibody (Ab124746) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant RBBP4 Antibody (Ab124746). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.