Chromosome 20 open reading frame 18 antibody | HBV associated factor 4antibody | HBV-associated factor 4 antibody | Heme-oxidized IRP2 ubiquitin ligase 1 antibody | Hepatitis B virus X associated protein 4 antibody | Hepatitis B virus X-associated protein
规格或纯度
ExactAb™, Validated, Carrier Free, 0.5 mg/mL
生化机理
Acts as an E3 ubiquitin-protein ligase, or as part of an E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteoasome. Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates. LUBAC conjugates linear polyubiquitin to IKBKG at 'Lys-285' and 'Lys-309' and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.
Post-translational: Auto-ubiquitinated. Auto-ubiquitination leads to degradation by the proteasome. Phosphorylated. In vitro, phosphorylation inhibits auto-ubiquitination activity.
RBCK1 Mouse mAb (Ab124756) - Western Blot All lanes: RBCK1 Mouse mAb (Ab124756) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 57 kDa Observed band size: 57 kDa The expression molecular weight observed is consistent with what has been described in the literature (PMID: 31209050, PMID: 27006117). The higher MW (57 kDa) band is the ubiquitylated form of RBCK1 (PMID: 31209050). Bands below 57 kDa are cleaved RBCK1 (PMID: 27006117). Exposure time: 65.7 s
ZJ25F0622839
危险品化学品经营许可证（带存储）