Rabbit anti Human RCC1 Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function: Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
Recombinant RCC1 Antibody (Ab124863) - Western Blot All lanes: Recombinant RCC1 Antibody (Ab124863) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa Observed band size: 47 kDa Exposure time: 10.0 s
Recombinant RCC1 Antibody (Ab124863) - Western Blot All lanes: Recombinant RCC1 Antibody (Ab124863) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa Observed band size: 47 kDa Exposure time: 20.0 s
Recombinant RCC1 Antibody (Ab124863) - IF/ICC Immunocytochemistry analysis of RCC1 (green) in HeLa using Recombinant RCC1 Antibody (Ab124863), and cells were counterstained with DAPI (blue).