Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein.
宿主种属
兔(Rabbit)
特异性
RGS4
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
KLH conjugated synthetic peptide derived from human RGS4 (AA 51-150).
阳性对照
WB: Mouse brain, Rat brain lysates. IHC: Mouse brain tissue.
RGS4 Antibody (Ab125221) - Western Blot All lanes: RGS4 Antibody (Ab125221) at 1/2000 dilution Samples: Lysates at 15 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 23 kDa Observed band size: 33, 22 kDa Exposure time: 100.0 s
RGS4 Antibody (Ab125221) - Western Blot All lanes: RGS4 Antibody (Ab125221) at 1/2000 dilution Samples: Lysates at 15 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 23 kDa Observed band size: 33 kDa Exposure time: 4.0 s
RGS4 Antibody (Ab125221) - IHC Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citratebuffer (pH 6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blockingbuffer (normal goat serum) at 37°C for 30 min, Antibody incubation with RGS4 Antibody (Ab125221) at 1/200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.