Rabbit anti Human RHOG Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function: Required for the formation of membrane ruffles during macropinocytosis. Required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry.
规格
产品名称
RHOG Antibody
别名
RHOG抗体 | RHOG 抗体
英文别名
Ras homolog family member G antibody | Rho G antibody | Rho related GTP binding protein RhoG precursor antibody | Rho-related GTP-binding protein RhoG antibody | RHOG antibody | RHOG_HUMAN antibody | RHOG | ARHG | ARHG antibody
规格或纯度
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
宿主种属
兔(Rabbit)
特异性
RHOG
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RhoG.
阳性对照
WB: Mouse spleen, K562, Jurkat and NIH/3T3 lysates. IHC: Human brain tissue.
RHOG Antibody (Ab125297) - Western Blot All lanes: RHOG Antibody (Ab125297) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 20 kDa Exposure time: 10.0 s
RHOG Antibody (Ab125297) - Western Blot All lanes: RHOG Antibody (Ab125297) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 20 kDa Exposure time: 5.2 s
RHOG Antibody (Ab125297) - IHC Immunohistochemical analysis of RHOG staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with RHOG Antibody (Ab125297) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.