ExactAb™,已验证 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human RIT1/RIT Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 1/500-1/2000
IHC: 1/50-1/300
Protein Function
Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation.
RIT1/RIT Antibody (Ab125431) - Western Blot
All lanes: RIT1/RIT Antibody (Ab125431) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 28 kDa
Exposure time: 19.4 s
RIT1/RIT Antibody (Ab125431) - IHC
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. RIT1/RIT Antibody (Ab125431) was diluted at 1/200 (4℃， overnight). Tris-EDTA, pH 9.0 was used for antigen retrieval. Secondary antibody was diluted at 1/200 (room temperature, 45 min).
RIT1/RIT Antibody (Ab125431) - IF/ICC
IF analysis of RIT1/RIT Antibody (Ab125431) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with RIT1/RIT Antibody (Ab125431) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
1/500-1/2000
1/200
1/50-1/300
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1113847 |
分析证书 | Ab125431 |
| ZJ24F1113848 |
分析证书 | Ab125431 |
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