RPL10A Antibody (Ab125777) - Western Blot All lanes: RPL10A Antibody (Ab125777) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/10000 dilution
Predicted band size: 25 kDa Observed band size: 26 kDa Exposure time: 22.0 s
RPL10A Antibody (Ab125777) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using RPL10A Antibody (Ab125777). Antibody was diluted at 1/200 (4° overnight). Tris-EDTA, pH9.0 was used for antigen retrieval. Secondary antibody was diluted at 1/200 (room temperature, 45min).
RPL10A Antibody (Ab125777) - IF/ICC IF analysis of RPL10A Antibody (Ab125777) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with RPL10A Antibody (Ab125777) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).